After natural/artificial insemination, pregnancy testing is important. Equine owner can get the pregnancy test done by the rectal palpation examination/ultrasonography/serum-based hormonal assay test. In view of the advantages and disadvantages of each technology, the farmers can choose any of the techniques given below.

• Old, economical and well established method for pregnancy diagnosis in mares
• Diagnose the pregnancy as early as 18^th day of conception and more precisely by 25^th to 30^th

• Visualization of the conceptus is possible from day 14 of pregnancy.
• Not used very frequently under field conditions as ultrasound scanner is costly and require expertise.

• ECG based ELISA for pregnancy diagnosis is simple and economical as it does not involve the transport of the animal and avoids extra stress.
• Effective in diagnosing the pregnancy between days 35 and 125 of gestation.